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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
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Fecha : |
29/09/2014 |
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Actualizado : |
25/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GIL, J.; GENOVESE, P.; SARTORE, I.; BIELLI, A.; MONTOSSI, F.; VIÑOLES, C. |
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Afiliación : |
J. GIL, Universidad de la República (UdelaR)/ Facultad de Veterinaria; P. GENOVESE, Universidad de la República (UdelaR)/ Facultad de Veterinaria; I. SARTORE, Universidad de la República (UdelaR)/ Facultad de Veterinaria; A. BIELLI, Universidad de la República (UdelaR)/ Facultad de Veterinaria; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA VIÑOLES GIL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
The leptin receptor is expressed in the Leydig cells of Merino rams, but its expression is not affected by long-term differences in the plane of nutrition |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Animal Production Science, 2014 v. 54, p. 981-986. |
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DOI : |
10.1071/AN12320 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 12 September 2012 / Accepted 26 September 2013 / Published online 19 December 2013. |
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Contenido : |
ABSTRACT.
We studied whether the long-term effects of nutrition on ram reproductive potential are mediated by changes in the expression and distribution of leptin receptor (Ob-Rb) in the testes. Thirty-two young Merino rams were allocated to a 2 × 2 factorial design, with either natural (NP) or improved pastures (IP), with (+S) or without (?S) supplementation. After 14 weeks of treatment, a blood sample was taken to measure leptin concentrations and rams were castrated. The weights of testicles (TW) and epididymides (EW) were recorded before tissue sampling for the detection of Ob-Rb expression by immunohistochemistry. TW was positively affected by the interaction IP × S, but not EW and bodyweight (BW), which were affected either by IP or S (P < 0.05). TW and EW were greatest (g) in IP+S rams (290.6 ± 40.1 and 57.6 ± 2.3), followed by IP?S (284.6 ± 61.4 and 52.5 ± 2.3), NP+S (282.6 ± 37.9 and 55.8 ± 2.3), and NP?S rams (208.5 ± 48.7 and 44.4 ± 2.3; P < 0.01). Expression of Ob-Rb was observed in the cytoplasm of Leydig cells, without any effect of treatments on the percentage of positive Leydig cells or on staining-intensity score. The serum leptin concentrations were similar among groups. The absolute volume of the seminiferous epithelium (mL) was affected by IP and S, as well as by their interaction (IP?S: 179.7 ± 13.1, IP+S; 174.9 ± 9.0; NP+S: 178.0 ± 10.2; NP?S: 120.8 ± 9.8; P < 0.05). We conclude that, under the conditions of the present study, the long-term improvement of nutrition promoted histological changes of increased spermatogenesis of Merino rams, but was not related to changes in the expression of the Ob-Rb receptor in the Leydig cell cytoplasm. MenosABSTRACT.
We studied whether the long-term effects of nutrition on ram reproductive potential are mediated by changes in the expression and distribution of leptin receptor (Ob-Rb) in the testes. Thirty-two young Merino rams were allocated to a 2 × 2 factorial design, with either natural (NP) or improved pastures (IP), with (+S) or without (?S) supplementation. After 14 weeks of treatment, a blood sample was taken to measure leptin concentrations and rams were castrated. The weights of testicles (TW) and epididymides (EW) were recorded before tissue sampling for the detection of Ob-Rb expression by immunohistochemistry. TW was positively affected by the interaction IP × S, but not EW and bodyweight (BW), which were affected either by IP or S (P < 0.05). TW and EW were greatest (g) in IP+S rams (290.6 ± 40.1 and 57.6 ± 2.3), followed by IP?S (284.6 ± 61.4 and 52.5 ± 2.3), NP+S (282.6 ± 37.9 and 55.8 ± 2.3), and NP?S rams (208.5 ± 48.7 and 44.4 ± 2.3; P < 0.01). Expression of Ob-Rb was observed in the cytoplasm of Leydig cells, without any effect of treatments on the percentage of positive Leydig cells or on staining-intensity score. The serum leptin concentrations were similar among groups. The absolute volume of the seminiferous epithelium (mL) was affected by IP and S, as well as by their interaction (IP?S: 179.7 ± 13.1, IP+S; 174.9 ± 9.0; NP+S: 178.0 ± 10.2; NP?S: 120.8 ± 9.8; P < 0.05). We conclude that, under the conditions of the present study, the long-term improvement... Presentar Todo |
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Thesagro : |
MERINO; NUTRICIÓN ANIMAL; OVINOS. |
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Asunto categoría : |
--
L51 Fisiología Animal - Nutrición |
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Marc : |
LEADER 02510naa a2200241 a 4500
001 1052570
005 2019-11-25
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1071/AN12320$2DOI
100 1 $aGIL, J.
245 $aThe leptin receptor is expressed in the Leydig cells of Merino rams, but its expression is not affected by long-term differences in the plane of nutrition
260 $c2014
500 $aArticle history: Received 12 September 2012 / Accepted 26 September 2013 / Published online 19 December 2013.
520 $aABSTRACT. We studied whether the long-term effects of nutrition on ram reproductive potential are mediated by changes in the expression and distribution of leptin receptor (Ob-Rb) in the testes. Thirty-two young Merino rams were allocated to a 2 × 2 factorial design, with either natural (NP) or improved pastures (IP), with (+S) or without (?S) supplementation. After 14 weeks of treatment, a blood sample was taken to measure leptin concentrations and rams were castrated. The weights of testicles (TW) and epididymides (EW) were recorded before tissue sampling for the detection of Ob-Rb expression by immunohistochemistry. TW was positively affected by the interaction IP × S, but not EW and bodyweight (BW), which were affected either by IP or S (P < 0.05). TW and EW were greatest (g) in IP+S rams (290.6 ± 40.1 and 57.6 ± 2.3), followed by IP?S (284.6 ± 61.4 and 52.5 ± 2.3), NP+S (282.6 ± 37.9 and 55.8 ± 2.3), and NP?S rams (208.5 ± 48.7 and 44.4 ± 2.3; P < 0.01). Expression of Ob-Rb was observed in the cytoplasm of Leydig cells, without any effect of treatments on the percentage of positive Leydig cells or on staining-intensity score. The serum leptin concentrations were similar among groups. The absolute volume of the seminiferous epithelium (mL) was affected by IP and S, as well as by their interaction (IP?S: 179.7 ± 13.1, IP+S; 174.9 ± 9.0; NP+S: 178.0 ± 10.2; NP?S: 120.8 ± 9.8; P < 0.05). We conclude that, under the conditions of the present study, the long-term improvement of nutrition promoted histological changes of increased spermatogenesis of Merino rams, but was not related to changes in the expression of the Ob-Rb receptor in the Leydig cell cytoplasm.
650 $aMERINO
650 $aNUTRICIÓN ANIMAL
650 $aOVINOS
700 1 $aGENOVESE, P.
700 1 $aSARTORE, I.
700 1 $aBIELLI, A.
700 1 $aMONTOSSI, F.
700 1 $aVIÑOLES, C.
773 $tAnimal Production Science, 2014$gv. 54, p. 981-986.
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| 2. |  | CABRERA, D. Densidad, conducción y poda en duraznero cv Junegold. In: INIA Las Brujas. Programa Fruticultura. Resultados experimentales en frutales de carozo (durazneros y ciruelos), 14 setiembre 1994. Canelones (UY): INIA Las Brujas, 1994. p. 14-15 (INIA Serie Actividades de Difusión ; 30) Participantes: Ing. Agr. Jorge Soria Baraibar, Ing. Agr. Alicia Feippe, Téc.Granj. Pablo Rodríguez Bruno.| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 4. | | CABRERA, D. Manejo del monte. ln: INIA (Instituto Nacional de Investigación Agropecuaria). Programa Fruticultura. Tecnología disponible para la producción de durazneros en la zona norte del país. Jornada de divulgación (Canelones, 9 junio 1994). Canelones (UY): INIA Las Brujas, 1994. p. 12-16 (INIA Serie Actividades de Difusión ; 16) "INIA Las Brujas; INIA Salto Grande"| Tipo: Actividades de Difusión |
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| 5. | | Cabrera, D. Manzano: portainjertos Montevideo (Uruguay): IICAPROCISUR, 1999. p. 136 (IICA PROCISUR Diálogo ; 54)| Biblioteca(s): INIA Salto Grande; INIA Treinta y Tres. |
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| 9. | | CABRERA, D. Raleo en ciruela cv. Golden Japan. In: INIA Las Brujas. Programa Fruticultura. Resultados experimentales en frutales de carozo (durazneros y ciruelos), 14 setiembre 1994. Canelones (UY): INIA Las Brujas, 1994. p. 17 (INIA Serie Actividades de Difusión ; 30) Participantes: Ing.Agr. Jorge Soria Baraibar, Tec.Granj. Pablo Rodríguez Bruno.| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 10. | | CABRERA, D. Raleo en ciruela cv Stanley. In: INIA Las Brujas. Programa Fruticultura. Resultados experimentales en frutales de carozo (durazneros y ciruelos), 14 setiembre 1994. Canelones (UY): INIA Las Brujas, 1994. p. 16 (INIA Serie Actividades de Difusión ; 30) Participantes: Ing. Agr. Jorge Soria Baraibar, Téc.Granj. Pablo Rodríguez Bruno.| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 12. | | CABRERA, D. Portainjertos para ciruelo: consideraciones para Uruguay. In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Fruticultura. Actualización Técnica en el Cultivo del Ciruelo. Seminario Regional, 27 mayo 2003, INIA Las Brujas, Canelones (Uruguay). Canelones (Uruguay): INIA Las Brujas, 2003. p. 94-98 (INIA Serie Actividades de Difusión ; 315)| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 13. | | CABRERA, D. Portainjertos de duraznero cv Rey del Monte. In: INIA Las Brujas. Programa Fruticultura. Resultados experimentales en frutales de carozo (durazneros y ciruelos), 14 setiembre 1994. Canelones (UY): INIA Las Brujas, 1994. p. 6-7 (INIA Serie Actividades de Difusión ; 30)| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 14. | | Cabrera, D. Peral: portainjertos Montevideo (Uruguay): IICAPROCISUR, 1999. p. 137 (IICA PROCISUR Diálogo ; 54)| Biblioteca(s): INIA Salto Grande; INIA Treinta y Tres. |
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| 20. | | Cabrera, D.; Rodríguez, P. Avances en portainjertos para peral Las Brujas, Canelones (Uruguay): INIA Las Brujas, 2008. p. 50-55 (INIA Serie Actividades de Difusión ; 544) "Programa Nacional de Investigación en Producción Frutícola; INIA Las Brujas"| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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